From a strain of actinomadura madurae, a new siderophore was isolated. Actinomadura madurae mycetomas, australasian journal. Taxonomic revision of the actinomycete genera actinomadura. Definition of actinomadura spinosa in the medical dictionary by the free dictionary. The type strains of 33 actinomadura, 2 excellospora, and 4 microtetraspora species, together with those of microbispora echinospora and nocardiopsis africana, were assigned to two aggregate groups that merited generic status given chemical, molecular and numerical taxonomic evidence. The genus actinomadura is one of four genera of actinobacteria that belong to the family. Peritonitis caused by actinomadura madurae in a patient on. This is to our knowledge the first reported genome of a humanpathogenic actinomadura species.
Mycetoma is a chronic, painless, slowly progressive, noncommunicable disease, but multiple family members, relatives and residents living in the same. Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. No bone involvement was discovered in xray examination. Both physiological characteristics and fatty acid composition differentiate this. Definition of actinomadura africana in the medical dictionary by the free dictionary. It affects dermal and subcutaneous tissues, with putative contiguous extension to muscles or bones. Both of the two major pigments which were observed on thinlayer chromatograms had rf values significantly greater than prodigiosin. Actinomadura madurae definition of actinomadura madurae.
Actinomadura meyerae osteitis following wound contamination. Actinomadura pulveracea definition of actinomadura. Hanafy a1, ito j, iida s, kang y, kogure t, yazawa k, yaguchi t, mikami y. Actinomadura species pathogenicity the main species of. Mycetoma is an infection involving cutaneous and subcutaneous tissues, fascia, and bone caused by soilinhabiting bacteria actinomyces or fungi eumycetes. An actinobacterium, designated strain dls70t, was isolated from a soil sample from the surface of a rock on the peak of darangshi oreum a volcanic cone in jeju, republic of korea.
During this infectious disorder, skin lesions are characterized by the presence of nodular grainlike structures that contain microcolonies of the causal agent surrounded by an abundant inflammatory infiltrate. Looking for online definition of actinomadura pulveracea in the medical dictionary. Complete remission occurred in 3 years with oral tetracycline. We describe a case of mycetoma caused by actinomadura pelletieri with simultaneous involvement of the spine, abdominal wall and retroperitoneal space in a man who had suffered from madura foot 10 years earlier. A 22yearold woman developed soft tender nodules on the right medial ankle and plantar foot at the beginning of pregnancy figure 1. Majority of actinomadura clinical isolates from sputa or. Traditional taxonomic methods plus contemporary fatty acid analysis techniques were used to establish the position of th.
Mycetoma is a chronic granulomatous infection caused by environmental fungi or bacteria. Strains received as actinomadura fell into two large aggregate groups. On the basis of data from this polyphasic study, strain dls70 t kctc 29224 t dsm 45941 t is considered to represent a novel species of the genus actinomadura, for which the name actinomadura darangshiensis sp. Scribd is the worlds largest social reading and publishing site. The aggregate taxa corresponded with the genera actinomadura and microtetraspora.
Nonmycetomic actinomadura madurae infection journal of clinical. Actinomadura madurae mycetomas actinomadura madurae mycetomas venugopal, pankajalakshmi v venugopal, taralakshmi v. A yokota eds identification manual of actinomycetes business center for academic societies japan tokyo 919. Actinomadura coerulea, actinomadura coeruleoviolacea, actinomadura cremea, actinomadura livida, actinomadura madurae, actinomadura malachitica, actinomadura pelletieri, and actinomadura verrucosospora. Jan 02, 2015 actinomycetoma may be due to actinomadura madurae, actinomadura pelletieri, streptomyces somaliensis, nocardia spp.
Eumycetoma is often due to pseudallescheria boydii scedosporium apiospermum, madurella mycetomatis. Numerical taxonomy of actinomadura and related actinomycetes. Pdf nonmycetomic actinomadura madurae infection in a patient. Madura foot caused by actinomadura madurae in a pregnant. Prodigiosinlike pigments from actinomadura nocardia. Actinomadura pelletieri mycetoma an atypical case with spine and abdominal wall involvement antonio cascio 1,2, giuseppe mandraffino 3, maurizio cinquegrani 3, demetrio delfino 4, rossella mandraffino 3, orazio romeo 5, giuseppe criseo 5, antonino saitta 3. A few whitish granules were detected in the oilylike discharge, the same as in the histologic examination. We report a rare case of actinomycetoma of the foot madura foot due to actinomadura madurae in a patient living in a temperate zone. We describe a case of actinomadura meyerae osteitis in a 49yearold caucasian woman who. Pdf actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of.
Actinomadura carminata 8 and actinomadura spadix 9 were transferred to the genera nonomuraea and actinoallo. Read actinomadura madurae mycetomas, australasian journal of dermatology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. On microscopic examination with h ematoxylin and eosin stain, these grains are purple and exhibit peripheral pink pseudofilaments figure 2. Nmr and ms analyses of 1 indicated high similarity with the siderophores named madurastatins.
Actinomadura spinosa definition of actinomadura spinosa. A new species of actinomadura, for which we propose the name actinomadura yumaensis, was isolated from a soil sample from yuma county, ariz. Twenty seven cases of actionomycotic mycetoma caused either by actinomadura madurae or actinomadura pelletierii have been described. One, actinomadura sensu stricto, contained actinomadura citrea, actinomadura coerulea, actinomadura coeruleoviolacea, actinomadura cremea, actinomadura livida, actinomadura madurae, actinomadura malachitica, actinomadura pelletieri, and actinomadura verrucosospora. A new species of the genus actinomadura which belongs to the actinomadura madurae group of goodfellow et al. This pathogen is one of the classical agents of actinomycotic mycetoma and is consistent with the organism visualized histologically. Nevertheless, deep sequencing recovered 748 reads 2. Actinomadura madurae is characterized by large, whiteyellow granules that can be seen with the naked eye mm. She immigrated to washington state from mexico 18 months prior to the onset of symptoms.
C nonmycetomic actinomadura madurae infection in patient. We describe a case of actinomycetoma caused by actinomadura mexicana in the caribbean region. Get a printable copy pdf file of the complete article 956k, or click on a page image. Infection enters through sites of local trauma eg, a cut or splinter, causing a granulomatous reaction. Nonmycetomic actinomadura madurae infection in a patient. It contains aerobic, grampositive, nonacidfast, nonmotile, chemoorganotrophic actinomycetes that produce welldeveloped, nonfragmenting vegetative mycelia and aerial hyphae that differentiate into surfaceornamented spore chains.
Characterization of madurastatin c1, a novel siderophore from actinomadura sp. Thirteen red strains of actinomadura nocardia pelletieri and three of a. If you continue browsing the site, you agree to the use of cookies on this website. It has not previously been reported to have caused invasive pulmonary or disseminated infection in humans. Cloning of the mdp gene cluster was confirmed by heterologous complementation of enediyne polyketide synthase pks mutants from the c1027 producer streptomyces globisporus and the neocarzinostatin producer. Oct 29, 2011 actinomadura stephania herrera juan david leal janet melo rodriguez slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising.
Characterization of the maduropeptin biosynthetic gene. The characteristics of this case were analysed and contextualized among those of other cases of mycetoma caused by other microorganisms. Both of the two major pigments which were observed on thinlayer chromatograms had r f values significantly greater than prodigiosin. They are characterised by subcutaneous granulomas and abscesses with in duration, formation of. Analysis of biopsy specimens revealed small whiteyellow grains extruded. The genus actinomadura is one of four genera of actinobacteria that belong to the family thermomonosporaceae. Numerical classification of actinomadura and nocardiopsis. Substrate mycelium was produced abundantly on most of the media tested. A yokota eds identification manual of actinomycetes business center for academic societies japan tokyo 9 19. Traditional taxonomic methods plus contemporary fatty acid analysis techniques were used to establish the position of this species. The polar lipids of actinomadura dassonvillei, actinomadura madurae, and actinomadura pelletieri were analysed by twodimensional thinlayer chromatography. Actinomycotic mycetoma caused by actinomadura madurae has been described with sporotrichosis like distribution along. Actinomadura madurae mycetomas, australasian journal of. The complex is a mixture of four factorsa 1, a 2, b 1, and b 2 that differ only in the structure of the acyl group.
Polar lipid composition in the classification of some. Here, we utilized deep sequencing of 16s rrna to implicate actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms. Nonmycetomic actinomadura madurae infection in a patient with. Neither actinomadura madurae nor actinomadura pelletieri strain was isolated, and. Mycetoma of the forearm due to actinomadura madurae. Pdf nonmycetomic actinomadura madurae infection in a. Majority of actinomadura clinical isolates from sputa or bronchoalveolar lavage fluid in japan belongs to the cluster of actinomadura cremea and actinomadura nitritigenes, and the description of actinomadura chibensis sp. Wo1999035285a2 hybridization probes which differentiate. Pdf actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. Actinomadura definition of actinomadura by medical dictionary. Mycetoma is a subcutaneous disease that can be caused by aerobic bacteria actinomycetoma or fungi eumycetoma. A clinicopathological study of actinomycotic mycetomas caused by actinomadura madurae and actinomadura.
While common in tropical and subtropical areas, mycetoma is rare in europe. Here we present the draft genome sequence of a member of the thermomonosporaceae, actinomadura madurae liidaj290, isolated from a human case of mycetoma. Spiral chains of spores with warty surface developed on the aerial hyphae. A case of mycetoma, with abscesslike lesions which appeared on the right forearm of a 43yearold male, is briefly reported. Cloning of the mdp gene cluster was confirmed by heterologous complementation of enediyne polyketide synthase pks mutants from the c1027 producer streptomyces globisporus and the neocarzinostatin producer streptomyces carzinostaticus using the. A daily dose of 4 g bactrin brought significant improvement. It can be caused by fungi eumycetoma or bacteria actinomycetoma. October 2006 majority of actinomadura clinical isolates from sputa or bronchoalveolar lavage fluid in japan belongs to the cluster of actinomadura cremea and actinomadura nitritigenes, and the description of actinomadura chibensis sp. Actinomadura madurae definition of actinomadura madurae by.
Nonmycetomic actinomadura madurae infection in a patient with aids. Like other actinomycetes, they are gram positive and in smears. Keratinocyte infection by actinomadura madurae triggers an. Draft genome sequence of actinomadura madurae liidaj290.
The polymers of the actinomadura species belonged to type ii and each of the strains of the three species. Molecular diagnosis of actinomadura madurae infection by 16s. Pdf micetoma actinomicotico por actinomadura madurae. Diagnosis is based on clinical manifestations, including swelling and deformity of affected areas, as well as the presence of granulation tissue, scars, abscesses, sinus tracts, and a purulent exudate that contains the microorganisms. The invention provides a method to differentiate between bacteria from the actinomadura madurae group of bacteria and the maduromycetes taxa using two nucleic acid probes that are complementary to a conserved region of the maduromycetes 16s rrna genes and to a conserved region of the actinomadura madurae group of bacteria 16s rrna genes, respectively. Maduraferrin, a novel siderophore from actinomadura madurae. One hundred and fiftysix actinomadura strains, marker strains of related taxa, and related isolates from bagasse and fodder were the subject of numerical phenetic analyses using 90 unit characters.
In response to infection with these organisms the body produces an inflammatory immune response in the skin. Maduromycosis, also known as mycetoma, is a progressive granulomatous infection involving the skin and subcutaneous tissues with potential to invade muscle and bone. Molecular diagnosis of actinomadura madurae infection by. The most common aetiological agents are nocardia brasiliensis and actinomadura madurae. Maduraferrin is the fe complex of an oligopeptide composed of salicylic acid, alanine, glycine, l. Morphology actinomadura species are nonmotile and nonsporing. Actinomadura species pathogenicity the main species of medical importance are a. Characterization of madurastatin c1, a novel siderophore.
Actinomadura definition of actinomadura by medical. Actinomycetoma is a syndrome of the skin characterized by chronic inflammation and lesions with nodular grainlike structures. Actinomadura madurae is an aerobic actinomycete which is best known. Peritonitis caused byactinomadura madurae in a patient on capd. We describe a case of actinomadura meyerae osteitis in a 49yearold caucasian woman who suffered a tibia open fracture. The isolate from the patients blood was subjected to dilutional antimicrobial. Mycetoma is a chronic infection that is slow to develop and heal. The biosynthetic gene cluster for the enediyne antitumor antibiotic maduropeptin mdp from actinomadura madurae atcc 39144 was cloned and sequenced. Actinomadura pelletieri mycetoma an atypical case with. Actinomycetes and non sporing anaerobes questions and answers pdf free download in microbiology mcqs,interview questions,objective questions,multiple choice.
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